Determination of VASA and IFITM3 Immunolocalization in Experimental Hyperthyroidism in Rat Ovaries at the Stage of Diestrus
Published: 2023-02-27
Page: 18-27
Issue: 2023 - Volume 6 [Issue 1]
Serpil Ünver Saraydın *
Histology and Embryology Department, Medicine Faculty, Sivas Cumhuriyet University, Sivas, Turkey.
Kübra Başol
Histology and Embryology Department, Medicine Faculty, Sivas Cumhuriyet University, Sivas, Turkey.
Dursun Saraydın
Chemistry Department, Science Faculty, Sivas Cumhuriyet University, Sivas, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Aims: Hyperthyroidism occurs due to overproduction of thyroid hormone. We aim to investigate the relationship of VASA and IFITM3 with hyperthyroidism by demonstrating immunolocalization at the stage of diestrus in hyperthyroid rat ovaries.
Materials and Methods: To induce hyperthyroidism, rats were given 250 ug/kg/day of L-thyroxine by subcutaneous injection for 21 days. The control group was a saline solution injected only. Ovarian tissues of euthanized rats were removed after cardiac blood was collected.
Results: It was determined that VASA is intensely expressed in oocytes and theca layers of growing follicles, and IFITM3 is strongly expressed in germinal epithelium and oocytes.
Conclusion: The intense expression of VASA is most likely due to the abundant ATP in the mitochondria of the oocyte cytoplasm. The effect of VASA and IFITM3 expressions in hyperthyroidic ovary compared to the control group shows the link between hyperthyroidism and follicle development.
Keywords: VASA, IFITM3, ovary, hyperthyroidism, diestrus